Biometric authentication device (100) comprising: an input unit for entering identification information of a user; an image capture unit (106) for capturing an image of a user's hand several times; a matching unit (124) to extract information from veins, based on the image, from the image captured by hand by means of the image capture unit (106) and execute a matching procedure of matching the vein information with data of veins provided previously associated with the identification information; a unit of determination data generation to acquire determination data specifying, based on the image of the hand, whether the image of the hand is acquired by capturing the living organism or not; and a determining unit (130) to authenticate the user if a matching result of the matching unit (124) based on the image of the hand demonstrates its normality and if the determination data demonstrates that the image of the hand is acquired by capturing the living organism, and not to authenticate any user otherwise; The determination data generation unit includes a measurement unit for measuring a thickness of the vein in a predetermined position based on the image of the hand, and the determination unit (130) authenticates the user if the result of coincidence of the coincidence unit (124) based on the image of the hand demonstrates its normality and if the thickness of the vein in the predetermined position that is measured by the measurement unit shows a variation based on the time between the images of the hand producing artificially a variation in blood pressure while the user does not authenticate otherwise.Dispositivo de autenticación biométrica (100) que comprende: una unidad de entrada para introducir información de identificación de un usuario; una unidad de captura de imagen (106) para capturar una imagen de una mano del usuario varias veces; una unidad de coincidencia (124) para extraer información de venas, basándose en la imagen, de la imagen capturada de la mano me