It is intended to provide a method of producing a fungal culture obtained by culturing a fungus in a liquid medium, which contains as a culture material at least one member selected from among cereals, beans, potatoes, amaranthus and quinua, wherein the speed of releasing a nutrient in the culture material into the culture system is controlled to thereby control the productivity of an enzyme (in particular, a starch digesting enzyme, a vegetable fiber digesting enzyme or a protease) of the fungal culture. Namely, a method of producing a fungal culture characterized by comprising culturing a fungus by using a liquid medium, which contains as a culture material at least one member selected from among cereals, beans, potatoes, amaranthus and quinua, while controlling the speed of releasing a nutrient in the culture material into the culture system to thereby control the enzyme productivity of the fungal culture.
