PROBLEM TO BE SOLVED: To provide vaccine which can be inexpensively and easily produced and has high efficiency and which is for Neospora caninum.SOLUTION: The invention relates to vaccine formulation comprising a synthetic protein in which an amino acid sequence of NcGRA7, a film penetration region is removed and which has arrangement in which an extracellular domain and intracellular domain are coupled, as an antigen, to Neospora infection disease of ruminant. The NcGRA7 is exhibited in a stage of tachyzoite, so that, the NcGRA7 has high efficiency to an intermediate host (ruminant) existing in a form of tachyzoite, so that, there is possibility of efficiently preventing abortion in a cattle due to Neospora caninum infection. In addition, since a film penetration region with high hydrophobic property is removed, the vaccine formulation can be easily and inexpensively produced.SELECTED DRAWING: Figure 1COPYRIGHT: (C)2019,JPO&INPIT【課題】簡易かつ安価に生産でき、有効性も高い、ネオスポラ・カニナムに対するワクチンの提供。【解決手段】NcGRA7のアミノ酸配列中、膜貫通性領域が除外され、細胞外ドメインと細胞内ドメインとが連結された配列を有する合成タンパク質を抗原として含有する、反芻動物のネオスポラ感染症に対するワクチン製剤。NcGRA7は、タキゾイトなどのステージで高発現するため、タキゾイトの形態で存在する中間宿主（反芻動物）に対して有効性が高く、牛などのネオスポラ・カニナム感染による流産を有効に予防できる可能性がある。又、疎水性の高い膜貫通性領域などが除外されているため、簡易かつ安価に生産できる有利性がある。【選択図】図１
