A collecting method for a root portion of a plant is disclosed, which comprises the steps of: (A) planting at least one plant; (B) covering a light-sheltering layer on the root portion of the plant and a region where the root portion of the plant grows to form a non-soil cultivation space under the light-sheltering layer so that the root portion of the plant grows in the non-soil cultivation space; and (C) lifting open the light-sheltering layer so as to immediately cut off part of the root portion of the plant and to access the cut root portion. Accordingly, a picker can conveniently and efficiently pick the root portion from the plant without destroying topsoil stability and jeopardizing plant life.一種植物根部的採集方法,包括下列步驟:A、種植至少一植物; B、覆蓋一遮光層在該植物之根部至該根部生長的區域上,該遮光層下方形成了一無土培養空間,以使該根部於該無土培養空間中生長;以及C、掀開該遮光層,即可迅速截斷該植物之根部的一部分,以取得被截下的根部。藉此,採收者可在不破壞表土安定暨危及植物的生命前提下,提高採收植物根部的便利性以及效率。