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New method for inducing dopamine-producing neural precursor cells
专利权人:
Eisai R&D Management Co., Ltd.;Kyoto University
发明人:
Takahashi, Jun,Doi, Daisuke,Samata, Bumpei,Sekiguchi, Kiyotoshi,Ono, Yuichi
申请号:
AU2014316100
公开号:
AU2014316100A1
申请日:
2014.09.04
申请国别(地区):
AU
年份:
2016
代理人:
摘要:
Provided is a method for manufacturing dopamine-producing neural precursor cells from pluripotent stem cells, the method comprising: (i) a step in which pluripotent stem cells are cultured adherently on an extracellular matrix in a culture solution containing a reagent selected from the group consisting of BMP inhibitors, TGFβ inhibitors, SHH signal stimulants, and FGF8 and GSK3β inhibitors; (ii) a step in which a substance than binds to Corin and/or a substance that binds to Lrtm1 is used to collect Corin and/or Lrtm1 positive cells from the cells obtained in step (i); and (iii) a step in which the cells obtained in step (ii) are cultured in suspension in a culture solution containing a neurotrophic factor.
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/

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