The invention relates to a deer antler wine for postponing senility and improving sleep, which is characterized in that the deer antler wine is made from 1to 15 parts by weight of pilose antler, 60 to 200 parts by weight of Chinese yam, 30 to 90 parts by weight of tuckahoe, 30 to 90 parts by weight of medlar, 10 to 40 parts by weight of ginseng, pilose asiabell root or American ginseng, 400 to 900 parts by weight of liquor and 100 to 450 parts by weight of purified water which serve as raw materials with the parts by weight:. The invention further provides the preparation method for deer antler wine. The deer antler wine of the invention is a new healthcare liquor with simple formula and good taste, and has the healthcare functions of postponing senility, improving sleep, etc. The preparation method of the deer antler wine of the invention is scientific and advanced, can effectively extract various active ingredients of the raw materials, remove impurities, and provide the prepared deer antler wine with excellent taste and unconspicuous medicinal odor.