A manufacturing method of two-chamber type combined container-syringe including: a vacuum plugging process (S2) of eliminating air bubbles from the dissolving solution of the dissolving solution-filled cartridge and sealing the dissolving solution with a middle stopper after the dissolving solution filling process (S1) a vacuum plugging process (S2) has a first cooling processing (S21) of cooling the inside of a vacuum plugging equipment in which a dissolving solution-filled cartridge is disposed to a cooling temperature that does not freeze the dissolving solution, a first decompression processing (S23) of decreasing a pressure inside the vacuum plugging equipment while the vacuum plugging equipment maintains the cooling temperature after the first cooling processing (S21), and a middle stopper pushing processing (S24) of pushing the middle stopper downward and bringing the middle stopper into contact with the dissolving solution after the first decompression processing (S23). The dissolving solution can be easily filled in a bubble-free state in the two-chamber type combined container-syringe.
