#$%^&*AU2014202292A120140522.pdf#####ABSTRACT The present invention relates to a method of preparing cells and in particular to a method of preparing breastmilk stem cells (BSCs) by isolation from breastmilk and subsequent culture. The invention further relates to BSCs prepared by the methods of the invention 5 and to methods and uses thereof. The invention has been developed primarily as a method for preparing and culturing BSC.