A dental floss holder. The dental floss holder includes a body having a spool case for holding a length of dental floss, a cover for the spool case, a neck extending from the spool case along a first axis, and first and second fingers extending outward from said neck at an arcuate angle. In an embodiment, the fingers may be parallel. A manually engageable clamp is provided with threaded fastener that engages a threaded nut having a compressing flange, so that the compressing flange works against a land provided on the outside of the cover. A pivot post is provided to prevent dental floss from becoming entangled with the threads of the fastener.