SHEVCHENKO ALEKSANDR ALEKSEEVICH,Шевченко Александр Алексеевич,SHEVCHENKO LJUDMILA VASILEVNA,Шевченко Людмила Васильевна,CHERNYKH OLEG JUREVICH,Черных Олег Юрьевич,DZHAILIDI GEORGIJ ANASTASOVICH,Джаил
申请号：
RU2013131347/15
公开号：
RU0002531054C1
申请日:
2013.07.08
申请国别(地区):
RU
年份:
2014
代理人:
摘要：
FIELD: medicine.SUBSTANCE: invention refers to veterinary science and aims at the preventing pseudomonosis and rabbit viral haemorrhagic disease. As antigens, the vaccine contains a pure culture cell suspension of the pseudomonosis agent Pseudomonas aeruginosa and the rabbit viral haemorrhagic disease agent produced by sampling the involved organs of dead rabbits from a local epizootic centre. The suspension is prepared with the differential diagnostic media inoculated and pure agent cultures recovered. The growth procedure is performed separately in beef-extract broth of Pseudomonas aeruginosa with glucose in the concentration of microbial cells 4-5 bn in 1 cm3. The 10-15% rabbit liver suspension is prepared after being infected with the rabbit viral haemorrhagic disease, with an activity of not less than 103.0 LD50/cm3. This is mixed in equal ratios of formalin and aluminium hydroxide in the following proportions, wt %: pure culture cell suspension of the pseudomonosis agent Pseudomonas aeruginosa recovered from the local epizootic centre in the nutrient medium with a titre of 4-5 bn microbial cells in 1 cm3 38.0-41.5, the rabbit liver suspension with the rabbit haemorrhagic disease virus recovered from the local epizootic centre in normal saline with an activity of not less than 103.0-4.0 LD50/cm3 - 38.0-41.5, glucose - 2.0-1.0, formalin - 2.0-1.5, aluminium hydroxide - the rest.EFFECT: using the declared invention enables higher specificity and efficacy of the associated pseudomonosis and rabbit viral haemorrhagic disease with no negative side effect on the animals.1 tbl, 5 exИзобретение относится к области ветеринарии и предназначено для профилактики псевдомоноза и вирусной геморрагической болезни кроликов. Вакцина в качестве антигенов содержит суспензию клеток чистой культуры возбудителя псевдомоноза Pseudomonas aeruginosa и вируса геморрагической болезни кроликов, полученных путем отбора пораженных органов от павших кроликов из местного эпизоотического очага
