SCALABLE METHODS FOR PRODUCING RECOMBINANT ADENO-ASSOCIATED VIRAL (AAV) VECTOR IN SERUM-FREE SUSPENSION CELL CULTURE SYSTEM SUITABLE FOR CLINICAL USE

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专利权人:

INC.;SPARK THERAPEUTICS

发明人:

QU, Guang,LU, Lin,WRIGHT, John Fraser

申请号：

SG11201804400S

公开号：

SG11201804400SA

申请日:

2016.12.01

申请国别(地区):

SG

年份:

2018

代理人:

摘要：

Methods and compositions for transfecting cells with plasmids are disclosed. In certain embodiments, methods and compositions are disclosed in which transfection efficiency is significantly increased by contacting the cells being transduced with polyethyleneimine (PEI) that is free of nucleic acid during the transfection process. Therapeutically useful adeno-associated viral vectors generated according to the disclosed methods and compositions are also disclosed.

来源网站：

中国工程科技知识中心

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http://www.ckcest.cn/home/