A process of detecting Mastitis in dairy animals using locally available liquid anionic surfactant is hereby disclosed. The process comprising the following steps: a) computing for the different dilution levels of liquid anionic surfactant by percentage depending on the type of dairy animal to test for dairy buffalo (40- 50 pcnt ), for dairy cattle and goat (20- 30 pcnt ) and distilled/purified to the desired volume of the test solution to be use； b) measuring the computed percentage of liquid anionic surfactant and distilled/ purified water； c) mixing the liquid anionic surfactant and the distilled/ purified water in a beaker； d) stirring the mixture gently to avoid formation of bubbles； e) transferring the mixture in a clean, empty and dry bottle with cover； f) leaving the mixture undisturbed for 1 to 2 hours before use； g) labeling the prepared test solution； h) washing and drying of the udder； i) removing about 4 streams of milk from the different quarters； j) stripping about 2ml of milk from the different quarters to the test paddle or cup； k) adding of the 2ml of the prepared test solution to the equal volume of milk stripped from the quarters； l) rotating and tilting the mixture from left to right for 10 to 15 seconds； and m) interpreting the macroscopic results obtained. Whereas, a distinct precipitate formed and milk become slightly viscous but no tendency of gel formation means a weak positive result； The mixture thickens immediately with some gelatinous formation formed marks a positive mastitis result； and rapid Gel formation formed and causes the exterior of mixture to become convex and a thick mass is form while doing swirling and titling and when mixture will be pour out, the whole mass formation will drop marks a serious or acute mastitis infection. Formation of gel involves binding of leukocytes due to anionic liquid surfactant on the milk.
