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A PROCESS OF DETECTING MASTITIS IN DAIRY ANIMALS USING LOCALLY AVAILABLE LIQUID ANIONIC SURFACTANT
专利权人:
PANGASINAN STATE UNIVERSITY
发明人:
GANGAN, Jennifer E.,WAMINAL, Yancy O.
申请号:
PH22019000753
公开号:
PH22019000753U1
申请日:
2019.06.19
申请国别(地区):
PH
年份:
2020
代理人:
摘要:
A process of detecting Mastitis in dairy animals using locally available liquid anionic surfactant is hereby disclosed. The process comprising the following steps: a) computing for the different dilution levels of liquid anionic surfactant by percentage depending on the type of dairy animal to test for dairy buffalo (40- 50 pcnt ), for dairy cattle and goat (20- 30 pcnt ) and distilled/purified to the desired volume of the test solution to be use; b) measuring the computed percentage of liquid anionic surfactant and distilled/ purified water; c) mixing the liquid anionic surfactant and the distilled/ purified water in a beaker; d) stirring the mixture gently to avoid formation of bubbles; e) transferring the mixture in a clean, empty and dry bottle with cover; f) leaving the mixture undisturbed for 1 to 2 hours before use; g) labeling the prepared test solution; h) washing and drying of the udder; i) removing about 4 streams of milk from the different quarters; j) stripping about 2ml of milk from the different quarters to the test paddle or cup; k) adding of the 2ml of the prepared test solution to the equal volume of milk stripped from the quarters; l) rotating and tilting the mixture from left to right for 10 to 15 seconds; and m) interpreting the macroscopic results obtained. Whereas, a distinct precipitate formed and milk become slightly viscous but no tendency of gel formation means a weak positive result; The mixture thickens immediately with some gelatinous formation formed marks a positive mastitis result; and rapid Gel formation formed and causes the exterior of mixture to become convex and a thick mass is form while doing swirling and titling and when mixture will be pour out, the whole mass formation will drop marks a serious or acute mastitis infection. Formation of gel involves binding of leukocytes due to anionic liquid surfactant on the milk.
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