The present invention refers to a portable device for the collection of biological samples. The device comprises a receptacle (8) with a cover (2), which comprises a pierceable area (3) and may include a collection instrument (1), comprising a rod (4), and a collection arrangement at the end, which may include a solid matrix, for instance a sponge or a tampon (5). The receptacle (8) has a rounded or conical shaped bottom (10), at the center of which there is a conical or rounded-shaped projection (11), with one or more holes (12). The bottom of the receptacle (8) is in contact with a collector recipient through a system of close-aperture, which may comprise screw or a fitting system with protrusions (13). In this way, the device referred in the present invention allows for the collection, storage, transportation and separation of biological material without significant manipulation and without risk of cross-contamination.
