МЕРЛО Дональд Дж. (US),РАССЕЛЛ Шон М. (US),РЕТАЛЛАК Дайан (US),ВУСЛИ Аарон (US),МИД Том (US),НАРВА Кеннет Э. (US)
申请号：
RU2013108769/10
公开号：
RU2013108769A
申请日:
2011.07.29
申请国别(地区):
RU
年份:
2014
代理人:
摘要：
1. A plant transformation method comprising contacting a plant cell with an Agrobacterium strain containing at least one auxiliary pTi plasmid comprising a 14.8 KpnI fragment from pSBl and a pTi plasmid containing at least one disarmed T-DNA region moreover, the T-DNA region includes at least the right border of T-DNA and exogenous DNA adjacent to the border, where the plasmids contain different origin of replication. 2. The method of claim 1, wherein the 14.8 KpnI VirBCDG fragment isolated from pSBl in Agrobacterium strains has a RecA.3 deficiency. A plant transformation method comprising contacting a plant cell with an Agrobacterium bacterium containing a 14.8 KpnI VirBCDG fragment from pSBl and a pTi plasmid containing at least one disarmed T-DNA region, where a 14.8 KpnI VirBCDG fragment is integrated into the neutral site bacteria chromosome integration. 4. The method of plant transformation according to any one of paragraphs. 1 and 2, where the bacterium further comprises a plasmid containing a T-DNA region adjacent to at least one T-DNA boundary, the plasmid containing the origin of replication of the IncP.5 incompatibility group. The plant transformation method according to claim 3, wherein the bacterium further comprises a plasmid containing a T-DNA region adjacent to at least one Agrobacterium T-DNA border. A plant transformation method according to claim 3, wherein the Agrobacterium strain has a RecA.7 function deficiency. A plant transformation method according to claim 4, wherein the T-DNA region contains sequences of three or more genes. A plant transformation method according to claim 4, wherein the T-DNA region contains 25,000 or more base pairs. The plant transformation method according to claim 4, wherein the T-DNA region is sun1. Способ трансформации растения, включающий контактирование клетки растения со штаммом Agrobacterium, содержащим, по меньшей мере, одну вспомогательную плазмиду pTi, включающую фрагмент 14,8 KpnI из pSBl, и плазмиду pTi, со
