There is provided a culture apparatus which is capable of shortening a sterilization time and sterilizing the culture apparatus including a gas concentration measuring sensor, pipes connected to a gas concentration measuring device and every corner of a cultivating chamber. The culture apparatus includes a sterilizing gas generator 42 that supplies sterilizing gas into a cultivating chamber 4. The sterilizing gas generator 42 atomizes a sterilizer solution (oxygenated water) by means of an ultrasonic vibrator 46. The culture apparatus further includes an ultraviolet generator (ultraviolet lamp 50) that irradiates gas in the cultivating chamber 4 with an ultraviolet ray. The culture apparatus further includes a door (outer door 14) for blocking an opening of the cultivating chamber 4 in a free-opening/closing manner and a locking device 52 for prohibiting the door (outer door 14) from being opened. The culture apparatus further includes a controller 60 for controlling the locking device 52 to prevent the door (outer door 14) from being opened from start of the sterilization process to end of the decomposition process.