MACDONALD LYNN,STEVENS SEAN,GURER CAGAN,MEAGHER KAROLINA A.,MURPHY ANDREW J.
申请号：
NZ62797713
公开号：
NZ627977A
申请日:
2013.02.01
申请国别(地区):
NZ
年份:
2016
代理人:
摘要：
Disclosed is a rodent comprising one or more human VL gene segments and one or more human JL gene segments operably linked to a non-human immunoglobulin heavy chain constant region gene, wherein the rodent expresses one or more rodent ADAM6 proteins or functional fragments thereof from an inserted nucleic acid sequence. Also disclosed is a non-human animal whose genome comprises: (a) an insertion of one or more human VL gene segments and one or more human JL gene segments upstream of an non-human immunoglobulin light chain constant region gene, wherein the one or more human VL gene segments and one or more human JL gene segments are operably linked to the non-human immunoglobulin light chain constant region gene, (b) an insertion of one more human VL gene segments and one or more human JL gene segments upstream of an non-human immunoglobulin heavy chain constant region gene, wherein the one or more human VL gene segments and one or more human JL gene segments are operably linked to the non-human immunoglobulin heavy chain constant region gene, and (c) an inserted nucleic acid sequence that encodes a rodent ADAM6 protein or a functional fragment thereof, wherein the rodent ADAM6 protein or functional fragment thereof is expressed from the inserted nucleic acid sequence, so that B cells of the non-human animal express antibodies that each include two immunoglobulin light chains paired with two immunoglobulin heavy chains, wherein each light chain comprises a human light chain variable domain and a non-human light chain constant domain and each heavy chain comprises a human light chain variable domain and a non-human heavy chain constant domain.
