A microdialysis system (100) is disclosed, comprising a microdialysis probe (30) insertable or inserted into tissue of a subject (40), a sensor module (50) configured to receive at least one fluid flow and sense at least one substance in the received at least one fluid flow, at least one fluid flow transport device configured to selectively convey a flow of fluid to the microdialysis probe (30), and at least one fluid flow path (60) for flow of fluid to the sensor module (50). The at least one fluid flow transport device is configured to selectively convey a flow of fluid including at least one reagent so as to join with the at least one fluid flow path (60) for flow of fluid to the sensor module (50) via at least one fluid flow connector (65； 70) for joining flow of fluid including at least one reagent with the at least one fluid flow path (60) for flow of fluid to the sensor module (50). The at least one fluid flow transport device is further configured to selectively convey a flow of calibration fluid so as to join with the at least one fluid flow path (60) for flow of fluid to the sensor module (50) via at least one fluid flow connector (65； 80) for joining flow of calibration fluid with the at least one fluid flow path (60) for flow of fluid to the sensor module (50). Each of the at least one fluid flow connector (65； 70, 80) is arranged and/or configured so as permit the joining of the respective flows with the at least one fluid flow path (60) for flow of fluid to the sensor module (50) without requiring any moving parts, e.g., by being implemented as one or more valveless fluid flow connectors.
