ASAHI KASEI MEDICAL CO.; LTD.;ASAHI KASEI MEDICAL CO., LTD.;旭化成メディカル株式会社
发明人:
UDA Miwako,烏田 美和子,ABE Hiroichi,阿部 博一,烏田 美和子,阿部 博一
申请号：
JPJP2013/064107
公开号：
WO2013/176140A1
申请日:
2013.05.21
申请国别(地区):
JP
年份:
2013
代理人:
摘要：
The purpose of the present invention is to provide a high-concentration protein solution production method for producing a protein-rich solution by concentrating a dilute protein such as ascetic fluid, said method enabling the production of a protein-rich solution having a high protein concentration without undergoing the deterioration in concentration efficiency caused by clogging or without employing an additional treatment time such as a time for an additional concentration step.The present invention is a method for producing a high-concentration protein solution, which comprises a first step of allowing a low-concentration protein solution stored in a storage container to pass through a polysulfone-based hollow-fiber-membrane-type filter for ascetic fluid concentration use, which is a filter having ultrafiltration performance of 85 to 150 mL/min/200 mmHg and having a hydrophilic polymer attached thereto, from the storage container via a circuit and discharging a filtrate through a filtration-side outlet port of the filter and, at the same time, discharging a high-concentration protein solution through an outlet port of the filter, and a second step of collecting the high-concentration protein solution that has been discharged through the outlet port of the filter into a collection vessel, wherein the first step comprises a first substep of allowing the low-concentration protein solution to pass through the filter for ascetic fluid concentration use at a first flow rate and a second substep of allowing the low-concentration protein solution to pass through the filter for ascetic fluid concentration use at a second flow rate that is higher than the first flow rate at a time point at which a portion having a predetermined volume or more of the total volume of the low-concentration protein solution is discharged.La présente invention a pour but de fournir un procédé de production de solution de protéine à concentration élevée pour produire une solution riche en pr
