Methods and systems for calibrating an eye-brain interface (EBI) system controlled on the basis of eye movements and brain waves according to one embodiment of the present invention are disclosed. A method includes: providing an eye-brain calibration (EBC) interface for calibrating eye movements and brain waves simultaneously, wherein the EBC interface comprises a visual object and instructs a user to gaze into the visual object in a particular cognitive state； acquiring eye movements and brain waves of the user for the visual object included in the EBC interface； mapping the visual object and eye movements of the user； and mapping the particular cognitive state instructed to the user and brain waves of the user.
