Disclosed are a method for destroying cellular mechanical homeostasis and the use thereof, especially disclosed is a method for destroying cellular mechanical homeostasis by using a Myosin inhititor thereby leading to cell softening and reduction of fibrosis in tissues and organs. Also disclosed are a method for promoting regeneration and repair of tissues and organs and the use thereof. The Myosin inhibitor can destroy the homeostasis of the cellular mechanical stress system, reduce the rigidity of tissues and organs in a pathological state, and stimulate stress and regeneration reactions similar to those in regeneration processes in lower organisms, and can use the characteristics to greatly inhibit fibrosis during the organ damage and to promote the regeneration and repair of tissues and organs, and meanwhile can use stress response to greatly improve the ability of cellular genetic repair. The Myosin inhibitor is preferably (-)-Blebbistatin or a derivative thereof (-)-Blebbistatin O-Benzoate. The method requires only a single small molecule or single factor treatment, and is simple in operation and good in repeatability, and can be used as a new method to stimulate stress response, to stimulate a regeneration reaction, to improve the ability of cellular genetic repair and/or to promote regeneration and repair of tissues and organs.L'invention concerne une méthode pour détruire l'homéostasie cellulaire mécanique et son utilisation, en particulier une méthode pour détruire l'homéostasie cellulaire mécanique à l'aide d'un inhibiteur de myosine pour conduire ainsi au ramollissement des cellules et à la réduction de la fibrose dans les tissus et les organes. L'invention concerne également une méthode pour favoriser la régénération et la réparation des tissus et des organes et son utilisation. L'inhibiteur de myosine peut détruire l'homéostasie du système de stress cellulaire mécanique, réduire la rigidité des tissus et organes à l'état pathologique, et stimuler le st
