The fluorescent observation apparatus (1) of the present invention is a light source section (3) for irradiating a living tissue (x) with an illumination light (L) containing excitation light, and a return light image generating section (61) for taking a return light of an illumination light (L) from a biological tissue (x) and generating a return light image (G1) A fluorescent image generation unit (62) that produces a fluorescent image (G2) by photographing fluorescence from the tissue (x), and the illumination light (L) is visible light which does not include at least a portion of the wavelength region of 490 nm to 540 nm, and the excitation light is It has a portion of the wavelength of illumination light (L) and generates fluorescence of wavelengths included in at least a portion of the wavelength region.本発明の蛍光観察装置(1)は、生体組織(X)に励起光を含む照明光(L)を照射する光源部(3)と、生体組織(X)からの照明光(L)の戻り光を撮影して戻り光画像(G1)を生成する戻り光画像生成部(61)と、生体組織(X)からの蛍光を撮影して蛍光画像(G2)を生成する蛍光画像生成部(62)とを備え、照明光(L)が、490nm~540nmのうち少なくとも一部の波長領域を含まない可視光であり、励起光が、照明光(L)の一部の波長を有し、前記少なくとも一部の波長領域に含まれる波長の蛍光を発生させる。
