FRYER, BENJAMIN M.,弗莱尔 班杰明,弗萊爾 班傑明,弗莱尔 班杰明,弗萊爾 班傑明
申请号：
TW107105801
公开号：
TW201843431A
申请日:
2018.02.21
申请国别(地区):
TW
年份:
2018
代理人:
摘要：
A method for detecting biological activity in a biological indicator comprises the steps of providing a known quantity of spores containing a fixed number of copies of an enzyme and a liquid growth medium comprising substrates of the enzyme. The enzyme substrates have a first emission spectrum, and are configured to be converted by the enzyme to substrate derivatives having a second emission spectrum. The spores are exposed to the liquid growth medium, and the liquid growth medium is measured for the second emission spectrum, and either no change in the second emission spectrum or a linear increase in the second emission spectrum, is detected as a function of time.一種用於偵測一生物指示器中之生物活性的方法，其包含提供含有一酶之一固定數目的複製物之一已知量的孢子及提供包含該酶的受質之一液體生長介質的該等步驟。該等酶受質具有一第一發射光譜，並經組態以由該酶轉變為具有一第二發射光譜的受質衍生物。使該等孢子暴露於該液體生長介質，及測量該液體生長介質的該第二發射光譜，並偵測該第二發射光譜中無改變或該第二發射光譜中的一線性增加的其中一者隨時間之變化。
