Devices, systems and methods for scaling the size and/or position of a marker on a magnified image of an object. In preferred embodiments, the object is an eye that is undergoing laser eye surgery. The eye is viewed through a magnification system or microscope and an image of the eye is presented on a display. One or more markers are present on the image, each identifying a specific target location or landmark on the eye. When a desired magnification setting is selected, the image is scaled accordingly. In addition, one or more of the markers is scaled in size and/or position to reflect the magnification setting. This allows the marker to maintain identification of the target location while reflecting the selected magnification level.