Bryan W. Kluttz,Geoff A. McKinley,Fabio Gennari,Michel Guy,Christopher Cotter,Luigi Catazariti,Louis Graziano,Bruno Colin,Cecile Paris,Jacque Dachaud
申请号：
US12924351
公开号：
US09562260B2
申请日:
2010.09.24
申请国别(地区):
US
年份:
2017
代理人:
摘要：
An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature. A pneumatic system is also provided that assists in fluid transfer of a reaction solution from the first chamber to the second chamber.
