Murphy, Andrew J.,Stevens, Sean,Gurer, Cagan,MacDonald, Lynn,Hosiawa, Karolina A.
申请号：
HRP20171666
公开号：
HRP20171666T1
申请日:
2017.10.31
申请国别(地区):
HR
年份:
2017
代理人:
摘要：
Genetically modified mice are provided that express human » variable (hV») sequences. The mice provided include a mouse comprising: (a) an endogenous heavy chain allele comprising an unrearranged human V H , an unrearranged human D H and an unrearranged human J H gene segment； and (b) an endogenous » (lambda) light chain allele comprising an unrearranged human V» and an unrearranged human J» gene segment. The mice provided include mice that express hV» sequences from an endogenous mouse » light chain locus, mice that express hV» sequences from an endogenous mouse º light chain locus, and mice that express hV» sequences from a transgene or an episome wherein the hV» sequence is linked to a mouse constant sequence. Mice are provided that are a source of somatically mutated human » variable sequences useful for making antigen-binding proteins. Compositions and methods for making antigen-binding proteins that comprise human » variable sequences, including human antibodies, are provided.
