PROBLEM TO BE SOLVED: To provide a cell cleaning liquid that improves a cell survival rate after cleaning, and a method for cleaning a cell using the same, and a cell-containing composition with a high cell survival rate and a method of preparing the same.SOLUTION: The present invention provides a cell cleaning liquid with an osmotic pressure ratio of 2 or more and 15 or less a method for cleaning a cell that includes a cell cleaning step for cleaning a cell using the cell cleaning liquid with an osmotic pressure ratio of 2 or more and 15 or less and a method of preparing a cell-containing composition and a cell-containing composition that contains cells and zinc with a concentration of 100 ng/mL to 400 ng/mL or more.SELECTED DRAWING: NoneCOPYRIGHT: (C)2018,JPO&INPIT【課題】洗浄後の細胞生存率が改善される、細胞洗浄液、及びこれを用いた細胞の洗浄方法、並びに細胞生存率の高い細胞含有組成物及びその調製方法を提供すること。【解決手段】浸透圧比が2以上15以下の細胞洗浄液、浸透圧比が2以上15以下の洗浄液を用いて細胞を洗浄する細胞洗浄工程を含む細胞の洗浄方法及び細胞含有組成物の調製方法、並びに細胞及び100ng/mLから400ng/mL以上の濃度の亜鉛を含む細胞含有組成物である。【選択図】なし