A kind of 3 D positioning equipment of Minimally Invasive Surgery, being includes: a pedestal, two angle positioning components, a locating rack, a slide positioning component, a pair of of hyte part, a contraposition adjustment component and a trimming assembly. Wherein,The pedestal includes two first frame bodies and two second frame bodies; Each angle positioning component system is pivoted respectively on the articulated section of each first frame body of what; The both ends system of the locating rack respectively with each angle positioning component Joint; The slide positioning component system can slide in the sliding rail portion of the fixed what locating rack according to predetermined lesion orientation; On adjustable fixed what second frame body of the contraposition component system; Contraposition adjustment component group is set on the what contraposition component; The trimming assembly system group is set on what contraposition adjustment component,Use so that Minimally Invasive Surgery more accurate positioning,Mode of operation is more simple and easy with light structure and other effects.一種微創手術的立體定位裝置,係包括:一座體、兩角度定位組件、一定位架、一滑座定位組件、一對位組件、一對位調整組件與一微調組件。其中,該座體包括兩第一架體與兩第二架體;各角度定位組件係分別樞設於各第一架體的樞接部上;該定位架的兩端係分別與各角度定位組件相固接;該滑座定位組件係可依預定病灶方位滑移固定於該定位架的滑軌部上;該對位組件係可調整固定於該第二架體上;該對位調整組件組設於該對位組件上;該微調組件係組設於該對位調整組件上,藉以使得微創手術定位更精準、操作方式更為簡單便利與結構輕巧等功效。