The present disclosure provides novel compositions and methods for the production and use of Agrobacterium tumefaciens strains (for example LBA4404) that are deficient in RecA activity relative to the parent strain. Combinations with other gene-deficient-strains of Agrobacterium tumefaciens are also disclosed. Specifically, two exemplary s recA minus strains, UIA777 where chloramphenicol resistant gene disrupting the recA gene and UIA770 where kanamycin resistant gene disrupting the recA gene are provided. The present disclosure provides novel compositions and methods for the production and use of Agrobacterium tumefaciens strains (for example LBA4404) that are deficient in RecA activity relative to the parent strain. Combinations with other gene-deficient-strains of Agrobacterium tumefaciens are also disclosed. Specifically, two exemplary s recA minus strains, UIA777 where chloramphenicol resistant gene disrupting the recA gene and UIA770 where kanamycin resistant gene disrupting the recA gene are provided. The disclosure relates to compounds specific for IL23A and TNF-alpha, compositions comprising the compounds, and methods of use thereof. Nucleic acids, cells, and methods of production related to the compounds and compositions are also disclosed.La presente descripción provee composiciones y métodos novedosos para la producción y uso de cepas de Agrobacterium tumefaciens (por ejemplo LBA4404) que son deficientes en la actividad de RecA con relación a la cepa progenitora. También se describen combinaciones con otras cepas deficientes de gen de Agrobacteriurn tumefaciens. Específicamente, se proveen dos cepas recA-, UIA777 en la cual el gen resistente a cloramfenicol interrumpe al gen recA y UIA770 en la cual el gen resistente a kanamicina interrumpe el gen recA.
