A hazardous drug deactivation wipe kit 100 includes a first pouch 102 having a one-way valve 208 coupled to an end thereof, a second pouch 104, and a third pouch 106. The first pouch 102 contains a wipe 209 saturated in a hypochlorite solution, the second pouch 104 contains a wipe saturated in thiosulfate solution, and the third pouch 106 contains a wipe saturated in isopropyl alcohol solution. The deactivation wipe kit may be used in a clean room to deactivate most hazardous drugs on a work surface.
