The present invention is related generally to the field of the production of antimicrobial cotton fabric(s). Particularly, the Invention provides a process for the production of antimicrobial cotton fabric(s) by using radiolytically synthesized nano silver colloidal particles. The process significantly achieves high antimicrobial efficacy with very low loading of 10 nanosilver colloidal particles on cotton fabric(s) and also exhibits reduced amount of leaching of nanosilver colloidal particles from the coated anti microbial cotton fabric(s).