PONATH, Paul D.,RINGLER, Douglas J.,JONES, S.,NEWMAN, Walter,SALDANHA, José,BENDIG, Mary M.
申请号：
ES97937148
公开号：
ES2262186T5
申请日:
1997.08.06
申请国别(地区):
ES
年份:
2015
代理人:
摘要：
The present invention relates to humanized immunoglobulins having binding specificity for ±4²7 integrin, comprising an antigen binding region of nonhuman origin (e.g., rodent) and at least a portion of an immunoglobulin of human origin (e.g., a human framework region, a human constant region). In one embodiment, the humanized immunoglobulin can compete with murine Act-1 for binding to human ±4²7 integrin. In a preferred embodiment, the antigen binding region of the humanized immunoglobulin comprises each of the complementarity determining regions of the light and heavy chains of the murine Act-1 antibody. The present invention further relates to a humanized immunoglobulin light chain or heavy chain, isolated nucleic acids comprising a sequence which encodes a humanized immunoglobulin or immunoglobulin chain of the present invention (e.g., a single chain antibody), constructs comprising a nucleic acid of the present invention, and host cells comprising a nucleic acid of the present invention useful in a method of preparing a humanized immunoglobulin. The humanized immunoglobulins can be used in diagnostic and therapeutic applications in humans, for example to control lymphocyte infiltration (including recruitment and/or accumulation) to mucosal tissue.
