Disclosed is a muscle synergy analysis which comprises detecting a myoelectric signal generated at each muscle of an antagonistic muscle pair group of a subject calculating, as time-series data, an antagonistic muscle ratio of an antagonistic muscle pair at a myoelectric signal level from a myoelectric signal detected during a predetermined motion by the subject and conducting multi variate analysis using the antagonistic muscle ratio at each time of the time-series data as a variable to calculate a principal component including at least a first principal component having high contribution to correlation.