The invention is intended to identify glutathione-S-transferase that exhibits the activities to metabolize and detoxify an isoxazoline derivative, such as pyroxasulfone. The invention provides a method for cultivating a transgenic plant into which a nucleic acid encoding a protein (a or b) below has been introduced in the presence of isoxazoline derivatives:(a) a protein comprising the amino acid sequence as shown in SEQ ID NO: 2； or(b) a protein comprising an amino acid sequence having 80% or higher identity to the amino acid sequence as shown in SEQ ID NO: 2 and having the activity of glutathione-S- transferase.本發明特定出對於派羅克殺草碸等異㗁唑啉衍生物顯示出代謝、解毒活性之麩胱甘肽-S-轉移酶。一種轉形植物之栽培方法，其特徵在於：於異㗁唑啉衍生物之存在下栽培導入有編碼以下(a)或(b)之蛋白質之核酸的轉形植物。(a)包含序列編號2之胺基酸序列之蛋白質(b)具有相對於序列編號2之胺基酸序列具有80%以上之同一性之胺基酸序列，且具有麩胱甘肽-S-轉移酶活性之蛋白質
