James Apgar,Philip Lessard,R. Michael Raab,Binzhang Shen,Gabor Lazar,Humberto de la Vega
申请号：
US13508234
公开号：
US20130036517A1
申请日:
2010.11.05
申请国别(地区):
US
年份:
2013
代理人:
摘要：
A method of predicting an intein insertion site in a protein that will lead to a switching phenotype is provided. The method includes identifying a plurality of C/T/S sites within the protein selecting from the plurality of C/T/S/ sites those that are ranked 0.75 or higher by a support vector machine, within ten angstroms of the active site of the protein, and at or near a loop-β-sheet junction or a loop-α-helix junction. A method of controlling protein activity and hosts including proteins with controlled activity are also provided. Also, intein modified proteins and plants containing intein modified proteins are provided.
