МАКВИРТЕР Джон (US),МАКДОНАЛД Линн (US),МЕРФИ Эндрю Джей. (US)
申请号：
RU2014141536
公开号：
RU2014141536A
申请日:
2013.03.15
申请国别(地区):
RU
年份:
2016
代理人:
摘要：
1. A non-human animal comprising in its germline a genetically modified immunoglobulin heavy chain locus comprising the nucleotide sequence of unrearranged variable region of a human immunoglobulin heavy chain, wherein the nucleotide sequence of unrearranged heavy chain variable region comprises at least one histidine codon which are not encoded by the corresponding gene segment of the heavy chain variable region germline cheloveka.2. Non-human animal according to Claim. 1, where the non-human animal is a mlekopitayuschee.3. animal of claim nonhuman. 2 where the mammal is a rodent selected from the group consisting of mouse, rat and homyachka.4. Non-human animal according to Claim. 1, where the added or substituted histidine codon is in the gene segment of the human immunoglobulin heavy chain gene segment selected from Vcheloveka, human D gene segment, gene segment and Jcheloveka kombinatsii.5. Non-human animal according to claim. 4, wherein the gene segment is a human immunoglobulin heavy chain gene segment is selected from Vzarodyshevogo human type D gene segment of human embryonic type gene segment Jzarodyshevogo type human and kombinatsii.6. Non-human animal according to claim. 5, where the added or substituted histidine codon is located at least in one reading frame of the gene segment of the human immunoglobulin heavy chain that encodes a heavy chain variable domain selected from the N-terminal region, the hinge region 4, complementarity determining region 1 (CDR1),
