The multi-parameter performance assessment and conditioning system disclosed herein is designed to assess and condition the peak performance of an athlete by measuring various biophysical parameters and employing a proprietary software algorithm to produce a real-time assessment score. The score can be monitored by the athlete in real time, by a proprietary software on a computing system or on a wrist band, so that the user is aware of the effect that their body's behavior is having on their performance. The system comprises an electroencephalogram, an electromyogram, and a series of physiological monitors to track the user's biophysical parameters, and can be implemented in a static training environment, a kinetic training environment, or on the user's field of play.