The disclosed artificial insemination system comprises a cervical plug, catheter, and positioning tool. The cervical plug comprises a shield configured to cover an orifice, an arm attached to one side of the shield that is operable to be inserted into the cervical canal, and an insert member on the other side of the shield. A bore disposed within the cervical plug and extending through the insert member, shield, and arm is operable to receive the catheter used to deposit the semen sample. The positioning tool comprises a handle with a stem extending longitudinally therefrom and terminating at a bracket that is operable to receive the insert member and catheter and assist in preventing the cervical plug from becoming dislodged when the catheter is removed. A further object of this invention is the process by which the disclosed system is used to increase the efficacy of artificial insemination.