The present invention relates to a method for promoting hair growth and preventing hair loss of a patient, a composition therefor, and a method for preparing the composition. More particularly, the method for promoting hair growth and preventing hair loss of a patient is characterized in that a composition containing an effective amount of an active ingredient comprising bFGF is intradermally or subcutaneously administered into a lesion of a patient suffering from hair loss. The method for promoting hair growth and preventing hair loss of a patient, the composition therefor, and the method for preparing the composition of the present invention have the effects that new blood vessels are generated in a lesion to thereby increase the supply of blood, and growth of fiber tissues and tissue regeneration are promoted to thus thicken the skin tissue of a lesion.