The present invention provides a blood bag sterile connection method and a device used in the method. The device includes a motor (12), a wire box (1), a PLC controller (11) and two vascular clamps (10). A left scroll (3-1) and a right scroll (3-2) are arranged in the wire box (1), wherein a composite metal wire (4) on the left scroll (3-1) is connected with the right scroll (3-2). The method includes the following steps: 1) placing blood bag tubes above the wire box (1), clamping positions to be cut by using the vascular clamps (10), and simultaneously moving the two vascular clamps (10) in opposite directions 2) heating the composite metal wire (4), and cutting the blood bag tubes by using the composite metal wire (4) 3) after cutting the blood bag tubes, aligning the blood bag tubes to be connected 4) moving the two vascular clamps (10) towards each other, rolling away the used composite metal wire (4), and rolling out a new composite metal wire (4) 5) connecting the blood bag tubes by heating the composite metal wire (4), and simultaneously moving the two vascular clamps (10) towards each other and 6) returning the wire box (1) to the initial position, taking out the connected blood bag tubes, and returning the vascular clamps (10) to the initial positions.