Compositions and methods for inducing an immune response against extra-intestinal pathogenicEscherichia coli(ExPEC) are described. In particular, multivalent vaccines containingE. coliantigen polysaccharide covalently bound to a exotoxin A ofPseudomonas aeruginosa(EPA) carrier protein that can withstand multiple environmental stresses are described.描述了用於誘導針對腸外致病性大腸桿菌(ExPEC)的免疫應答之組成物和方法。具體地,描述了可以禁得起多種環境緊迫的包含共價結合至銅綠假單胞菌的外毒素A(EPA)載體蛋白的大腸桿菌抗原多醣之多價疫苗。