A multi-function cooling and thermo-sensitive rack for microbiology experiment includes horizontal bars (1), a base (3), positioning holes (4), connectors (5), a lateral frame (6), and connecting holes (7). The lateral frame (6) is a U-shaped frame and dimensionally identical to the base (3). The lateral frame (6) and the base (3) are connected through the connectors. Arms of the U-shaped frame of the lateral frame (6) are symmetrically provided with plural connecting holes (7) thereon. The horizontal bars (1) are horizontally fixed in the connecting holes (7). The multi-function cooling and thermo-sensitive rack further includes a temperature-displaying prompter (8) and N temperature sensors (9), or the horizontal bars (1) and/or the base (3) are thermochromic.