A method for preparing and purifying recombinant lipoprotein Ag473 ofNeisseria meningitidis(NM) group B isolates. The method can be used in large-scale production of vaccines forNeisseria meningitidis(NM) group B.本發明係關於B群腦膜炎球菌(NM)重組Ag473脂化膜蛋白之製造及純化方法。該方法可用於大量生產B群腦膜炎球菌(NM)疫苗。