THE BRIGHAM AND WOMEN'S HOSPITAL, INC.;DANA-FARBER CANCER INSTITUTE, INC.;NATIONAL UNIVERSITY OF SINGAPORE
发明人:
Alexander FEDERATION,Li CHAI,Hiro TATETSU,Daniel Geoffrey TENEN,James Elliot BRADNER
申请号：
US16567194
公开号：
US20200121721A1
申请日:
2019.09.11
申请国别(地区):
US
年份:
2020
代理人:
摘要：
Described herein are methods for genetically modifying expanded hematopoietic cells comprising obtaining a quantity of hematopoietic cells, culturing the quantity of hematopoietic cells in the presence of at least one histone deacetylase inhibitor (HDACi) and at least one growth factor to expand the hematopoietic cells, and contacting the expanded hematopoietic cells with a nucleic acid sequence, wherein the nucleic acid sequence modifies the quantity of expanded hematopoietic cells. Further described herein are compositions comprising the genetically modified hematopoietic cells produced by these methods.
