This invention discloses a kind of preparation methods of Antrodia cinnamomea extract； belong to edible and medical fungus applied technical field. The described method includes: extracting of dish-cultured A. cinnamomea fruiting bodies powder by using ethanol solution. Then the extraction solution was filtered and concentrated under reduced pressure to obtain crude extract. The crude extract was purified using macroporous adsorption resin in tank, then washed by different mix solvent and concentrated under reduced pressure to dry to obtain A. cinnamomea extract fraction I. Then the fraction I was washed by ethanol solution and concentrated under reduced pressure to dry to obtain A. cinnamomea extract fraction II. Then the fraction I was washed by ethanol solution and concentrated under reduced pressure and dry to obtain A. cinnamomea extract fraction II. Then the fraction II was washed by ethyl acetate solution and concentrated under reduced pressured to dry to obtain A. cinnamomea extract fraction III. This invention also provides the preparation method and pharmaceutical composition for the extract and composition of A. cinnamomea. The fractions or compositions of A. cinnamomea extract produced by present invention can be used for treating cancer and mitigate side effects of chemotherapy.本發明公開了一種牛樟芝萃取物的製備方法，屬於食藥用菌應用技術領域。所述方法包括：使用乙醇溶液萃取皿培式牛樟芝子實體粉末，過濾液減壓濃縮後得到粗萃取物；將粗萃取物用大孔樹脂吸附後置於長方形容器中，依次先用混合溶液洗脫，將洗脫液減壓濃縮、乾燥，得到牛樟芝萃取物的第一分萃物後，再用乙醇溶液洗脫，將洗脫液減壓濃縮、乾燥，得到牛樟芝萃取物的第二分萃物後，再用乙酸乙酯溶液洗脫，將洗脫液減壓濃縮、乾燥，得到牛樟芝萃取物的第三分萃物。本發明還提供了牛樟芝組合物的製備方法和醫藥組合物。本發明制得的牛樟芝萃取物的分萃物或牛樟芝組合物，可用於治療癌症及減輕化療副作用。
