The present invention provides for a composition to be applied in the treatment of borreliosis and a kit. The composition includes four groups of components with which group A) the components 1000-5000 mgs Cat's Claw, 1000-3000 mgs Krill Oil or Kalamarine Oil or combinations of it, 200-600 mgs Magnesium Malate, 3000-5000 mgs ester C, 100-300 mgs Alpha-Lipoic Acid, 25-75 mgs Pyridoxyl-5-Phosphate (vitamin B6), 6000-14000 IU Cholecalciferol (vitamin D3), 2000-4000 mgs He Shou Wu, 1200-2500 mgs Purple Clovers Tea, 10-50 mls parsley juice, the group B) 2000-4000 mgs Monolaurine, 400-800 mgs N-Acetylcysteine, 100-300 mgs Hyaluronic acid with BioCell collagens II, 1-5 drops Teasel Root, the group C) 10-70 drops Spilanthes Acmella and the group D) 500-1500 μg Cyancobalamin (vitamin B12). The composition and the kit is suitable to be applied in the treatment of borreliosis with which the components of the group A) are administered once daily, those of the group B) twice daily, those of the group C) three times daily and that of the group D) every 14 days.