Use of a mixture live cultures probiotic bacteria in a mixture with short chained fatty acids (SCFA), in a slow release form for the active ingredients, of various release structures and mechanisms, in particular in the form of a triglyceride matrix; in the diet of monogastric animals, in particular a human diet, for modulating intestinal bacterial flora as well as improving the acid-base and water-electrolyte balance of the gastrointestinal tract; in the manufacturing of a preparation for maintaining bacterial and acid-base homeostasis of the distal portion of the gastrointestinal tract of monogastric animals, including the human small intestine and colon.