The invention discloses a scheme for preparing some specific nanobody system and delivering nanobody medicine through woman's vagina as well as its preparation method and applications. Women's vagina is rich of capillaries and lymphatic vessels, vaginal permeability is greater than the rectum, mouth, and skin. Vaginal administration can not only local medication, but also systemic medication. Due to the unstable activity of traditional antibody or high molecular protein, large volume and other reasons, the biomedical administration mode is basically limited to the injection type. Nanobodies are the smallest units known to bind target antigens. The three-dimensional structure of nanobodies determines its relative stable biological activity, also nanobody has the characteristics of high temperature resistance, acid-base resistance, good water-solubility and strong tissue penetration. Thus, a wide range of applications of the Nanobody delivery system in disease treatment and women's health care can be achieved by optimizing carrier formulation to maintain and enhance the stability and tissue penetration of the nanobody, Vaginal nanobody formulations include: creams, crosslinked polyvinyl alcohol (PEG) hydrophilic gels, vaginal gels, vaginal capsules and tablets, vaginal suppositories, vaginal rings, and vaginal film. Nanobody vaginal administration can be used for specific nanobody in the local application, also can be used to penetrate the vaginal mucosa into the bloodstream to reach the preset lesion site to play a role. The vaginal delivery system includes specific nanobodies directly against different target sites of disease, such as local targets include: vaginal infections, endometriosis, uterine fibroids, and contraception, while systemic targets include inflammation and Rheumatism, cancer, viral bacteria, cardiovascular diseases, diabetes mellitus, Alzheimer's disease, but the target sites of the vaginal administration of the nanobody is not limited thereto. Th
