Method, system, apparatus, and computer program products for removing marker artifacts from a tomosynthesis dataset. In the method, a first plurality of projection images are acquired by tomosynthesis x-ray imaging, the first plurality of projection images containing at least one imaged representation of at least one alignment marker. In one aspect, the imaged representation of the at least one alignment marker on the first plurality of projection images is minimized to generate a second plurality of projection images. In another aspect, a plurality of tomographic images are reconstructed from the second plurality of projection images.
