The invention relates to a pharmaceutical composition comprising isometamidium chloride with a concentration of 1 % to 4 % (weight/volume), isopropylidene glycerol or glycerine formal with a concentration of 40 % to 95 % (weight/volume), a polyol with a concentration of 0 % to 50 % (weight/volume), 15-polyethylene glycol hydroxystearate with a concentration of 0.5 % to 7 % (weight/volume), in solution together with pharmaceutically acceptable carriers, for the treatment of trypanosomiasis in animals. The pharmaceutical composition is an injectable solution which is ready to use and can be administered intravenously, subcutaneously or intramuscularly to ruminants, equids and camelids. The composition can also include anti-parasitic agents, such as ivermectin, with a concentration of 1 % to 5 % (weight/volume) for the treatment of trypanosomiasis and the elimination of gastrointestinal and pulmonary parasites in animals.
