MARK DONOVAN,DOMINIQUE BERNARD,ISABELLE CASTIEL,VERONIQUE CHAUSSADE
申请号：
FR0654965
公开号：
FR2908784B1
申请日:
2006.11.17
申请国别(地区):
FR
年份:
2012
代理人:
摘要：
Use of polypeptide of amino acid sequence coded by a nucleic acid sequence comprising a fully defined 471 base pairs (SEQ ID NO: 1) sequence given in the specification or its analogue or its fragment or a nucleic acid sequence coding for the polypeptide, as tool for in vitro or ex vivo evaluation of epidermis condition, is claimed. Independent claims are included for: (1) a use of an antibody specifically binding to a polypeptide, as tool for in vitro or ex vivo evaluation of epidermis condition； (2) a in vitro or ex vivo evaluation process of epidermal condition comprising: determining the polypeptide or nucleic acids coding for the polypeptide in an epidermis sample and comparing the determined content with a reference value； and determining a biological activity of the polypeptide in a epidermis sample and comparing the determined value with a reference value； (3) a process for preparing an isolated skin reconstruction comprising contacting the polypeptide or a sequence of nucleic acid coding the polypeptide, or at least one agent modulating the expression or activity of the polypeptide or its interaction with a components of the extracellular matrix with cells to generate an isolated skin rebuilt, preferably keratinocytes； and (4) a non-therapeutic process to highlight an effect of a treatment liable to revert a skin disorder or for assessing the effect of a cosmetic process to improve the condition of dry skin and/or aged skin in an individual, where the assessing process comprise conducting an initial determination of a biological activity and/or expression of the polypeptide in a first skin sample taken from the individual, before the treatment and/or implementation of cosmetics process, conducting a second determination of the biological activity and/or the expression of the polypeptide in a second skin sample taken from the individual determined in previous, after treatment and/or implementation of cosmetic process, and comparing the first and second determ
